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This article was aimed to study the protection effects of Pa-Bing Formula No. 2 (PBFN-2) on neurons of substantia nigra in Parkinson's disease (PD) rats models in order to explore the possible mechanism of PBFN-2 in PD treatment. Rats were stereotaxically injected with 6-hydroxydopamine (6-OHDA) solution into the left stria-tum in two-site. Rat showed consistent right whirling and the number of rotation was more than 7 r·min-1 induced by APO in 30 min, then the rat was judged as PD model. A total of 14 rats modeled successfully were randomly di-vided into the model group and the treatment group. At the same time, the normal group and sham operation group were also established. Same volume of distilled water was given to the normal group, sham operation group and the model group. PBFN-2 (32.0 g·kg-1) was given to the treatment group for 4 weeks. Microscope was used to observe pathological changes of substantia nigra by nissl stain and changes of TH, Nrf2 and HO-1 immunohistochemical stain. The results showed that compared with the normal group, the nissl bodies were badly injured. Expressions of TH-positive cells were obviously reduced (P<0.01). The expression of Nrf2 nucleus protein and HO-1 protein were obviously increased in substantia nigra of PD rats in model group (P<0.01). Compared to the model group, PBFN-2 effectively increased nissl bodies in neuronal cells of substantia nigra of PD rats, and elevated the number of TH-immunoreactive cells in substantia nigra (P<0.05). The expressions of Nrf2 nucleus protein, HO-1 protein were significantly up-regulated (P<0.05). It was concluded that PBFN-2 had an obvious neuroprotection on the neuronal cells in substantia nigra of PD rats induced by 6-OHDA. The underlying mechanisms may be associated with regu-lation of Nrf2 nucleus protein and HO-1 protein expressions.
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Objective To analyse the clinical and pathological features of cystadenocarcinoma in the intrahepatic biliary tract. Methods The clinical features of 14 patients with cystadenocarcinoma in intrahepatic biliary tract were analysed in respect to the results of ultrasonic and CT scannings, as well as pathological examination. Results Among the 14 patients, 11 were females and 3 were males. The age of the patients ranged from 32 to 79 years, and mean age was 59.5. The obvious symptoms were right-upper abdominal pain and abdominal distention, and major physical signs were hepatomegaly with cystic feeling. The ultra sonographic and CT scanning revealed cystic space occupying lesions in the liver. According to the invasion or infiltration of carcinoma to the liver tissue, the pathology of cystadenocarcinoma in intrahepatic biliary tract could be categorized into two types: the invading and non-invading. There were 8 patients belonging to the first type and they died 9～37 months after the diagnosis or operation, while 6 patients with no invasion were alive after operation. Conclusion The clinical picture and pathology of cystadenocarcinoma in intrahepatic biliary tract were described. The pathological classification was important in prognosticating the outcome of the patient.
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cases of focal nodular heperplasia of the liver were studied with HE staining and immunohistochemistry. The patient population was composed of 12 males and 5 females, aged 13 to 71 years. The main symptom was abdomimal pain or discomfort. 9 patients were misdiagnosed as hepatic cell carcinoma (HCC) before operation. Histologically, 14 cases were of classical form, and 2 cases were of telangiectatic form, and 1 case mixed form. The classical form of FNH was defined by the presence of 4 characteristic histologic features: nodular central stellate scar, abnormal nodular architecture, malformed vessels, and cholangiolar proliferation. The causes of focal nodular hyperplasia of the liver were briefly discussed.
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To evaluating the imaging features and the pathological basis of low grade malignant cystic mesothelioma of the peritoneum (LGMCMP). In 14 cases of LGMCMP, radiographic imaging (X ray, CT, and MRI) was reviewed, and diagnosis was established by pathology. The majority of the patients were middle aged women. The main symptoms were abdominal pain or discomfort. CT and MRI revealed solitary cystic masses in 3 of them, multiple cystic nodules in 5, and diffuse cystic nodules in 6. It is therefore concluded that X ray, CT, and MRI are helpful in revealing the characteristics of LGMCMP and confirm the diagnosis.
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Objective To study the clinicopathologic features and prognosis of low-grade malignant cystic mesothelioma of the peritoneum (LGMCMP). Methods 25 cases of LGMCMP were analyzed retrospectively on their clinicopathologic features and immunohistochemical and electron microscopic findings, therapeutic approach and prognosis were also studied. Results Of 25 cases, 18 were females and 7 were males with a mean age of 39.6 years (range 16-84 years). According to their imaging characteristics, symptoms and physical signs, three morphological patterns of these tumors were found, i.e. localized cystic mass, multiple cystic nodules and diffuse cystic nodules. Immunohistochemical staining showed that the neoplastic cells were positive for CK, Vimentin, EMA, and anti-mesothelial cell antigen. Histochemically, the tumors were positive for CI but negative for HCI. Electron microscopic study of 11 cases revealed that the neoplastic cells had profuse slender microvilli on cell surface, large amount of microfilaments (tono-filaments) and abundant desmosomes. Conclusion LGMCMP is an uncommon neoplasm of low-grade malignancy, generally occurring in young women with distinct clinicopathologic features.
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Objective To study histological and cytological characteristics of malignant mesothelioma of the peritoneum by needle biopsy. Methods Needle biopsy specimens of 19 cases of malignant mesothelioma of the peritoneum were studied with pathological, cytological, histochemical and immunohistochemical staining examination. Results Of 19 cases, 12 were males and 7 were females, with a mean age of 58.1 years. Histopathlogical examination of needle biopsy specimens revealed that 10 of them were tubulopapillary. 3 undifferentiated. 1 signet ringoid cell, 1 small cell, 2 of low-grade malignant cystic cell and 2 of myofibroblastic cell types. 16 cases were positive by cytology smear but 3 negative. Immunohistochemical staining showed that the neoplastic cells of all cases were positive for keratin, vimentin, EMA, antimesothelial cell antigen and CI but negative for HCI. Conclusion The diagnosis of malignant mesothelioma of the peritoneum can be established by its clinical manifestation, combined with pathological, cytological, histochemical staining and immunohisitochemical staining of specimens obtained by ultrasound guided needle biopsy.
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Objective To analys the clinicopathological features and pathological diagnosis and differential diagnosis of the nasal neuroendocrine carcinomas (NEC). Methods 12 cases of nasal neuroendo crine carcinomas were studied with clinical datas and light microscopic examination and electron microscopic and immunohistchemical staining. Results The majority of the patients were middleaged. The patient population was composed of 10 meles and 2 females(sex ratio,5:1) with average age 39 6 years. On pathological examination,8 of them were small cell type neuroendocrine carcinoma,3 large cell type neuroendocrine carcinoma and 1 atypical ueuroendocrine carcinoma. Electron microscopy of 8 cases showed abundant neuroendocrine granules in the tumors. Immunohistochemical analysis showed that cells expressed EMA,CK,CgA,NSE and Syn Conclusions The key point for clinicopathological features of the NEC were discussed. Its differentila diagnosis includes malignant lymphoma, undifferentiatel carcinoma, malignant melanoma and olfactory neuroblastoma. Histological type of the tumor is important for clinical treatment plan and prognostic evaluation